Effects and Its Mechanism of Different Dental Alloys on Proliferation and Apoptosis of Fibroblast / 医学研究杂志

Objective To investigate the effects and its mechanism of different dental alloys on proliferation and apoptosis of fibroblast.Methods Mouse fibroblast L929 cells were divided into negative control group,gold alloy group,Ni Cr alloy group and copper alloy group.Cell proliferation was detected by MTT assay.Cells were cultured for 48h.Cell cycle and apoptosis rate were detected by flow cytometry.The expression of Bcl-2,Bax,cleaved caspase-3 protein were detected by Western blot.Results Cell proliferation rate had no significant difference in gold alloy group and Ni Cr alloy group and negative control group (P ＞ 0.05),while was significantly lower in copper alloy group than the negative control group (P ＜ 0.01).G1 phase,S phase and G2 phase cells between gold alloy group and the negative control group had no significant difference (P ＞ 0.05),while G1 phase cells in Ni Cr alloy and copper alloy group was significantly lower than negative control group.S phase and G2 phase cells were significantly higher than the negative control group (P ＜ 0.05 or P ＜0.01).Cell apoptosis rate and the expression of Bcl-2,Bax,cleaved caspase-3 protein between gold alloy group and negative control group had no significant difference (P ＞ 0.05).Cell apoptosis rate and the expression of Bax,cleaved caspase-3 protein between Ni Cr alloy and copper alloy group were significantly higher than the negative control group.Bcl-2 protein expression was significantly lower than the negative control group (P ＜ 0.01).Conclusion The gold alloy,Ni Cr alloy and copper alloy can influence proliferation and apoptosis of fibroblast.The gold alloy group is minimum.Different dental alloys leads to the possible mechanism of fibroblast apoptosis was regulation of Bcl-2,Bax,cleaved caspase-3 protein expression.

Primary Carnitine Deficiency and Cardiomyopathy

Carnitine is essential for the transfer of long-chain fatty acids from the cytosol into mitochondria for subsequent beta-oxidation. A lack of carnitine results in impaired energy production from long-chain fatty acids, especially during periods of fasting or stress. Primary carnitine deficiency (PCD) is an autosomal recessive disorder of mitochondrial beta-oxidation resulting from defective carnitine transport and is one of the rare treatable etiologies of metabolic cardiomyopathies. Patients affected with the disease may present with acute metabolic decompensation during infancy or with severe cardiomyopathy in childhood. Early recognition of the disease and treatment with L-carnitine may be life-saving. In this review article, the pathophysiology, clinical presentation, diagnosis, treatment and prognosis of PCD are discussed, with a focus on cardiac involvements.

Cardiac/coronary artery lesion in Kawasaki disease treated with different kind of intravenous immunoglobulin therapy in Shanghai from 1998-2008 / 临床儿科杂志

Objective To evaluate the effect of different kind of intravenous immunoglobulin (IVIG) therapy in treating Kawasaki disease (KD) and preventing cardiac consequences (coronary artery lesion, CAL). Methods A questionnaire form and guideline for KD diagnosis were sent to 50 hospitals providing pediatric medical care in Shanghai. The data from a total of 1 682 KD patients were collected. It included 1 064 males and 618 females from 1998 through 2008 in Shanghai. The average age of the KD patients was (2.57±2.33) years old (0.1-18.8 years).The patients had been divided into 6 groups for different IVIG therapy, which included 1 g/kg once, 2 g/kg once, 0.4-0.5 g/kg five times, 1 g/kg twice, 2 g/kg twice and others. SAS 6.12 software was used for statistical analysis. Results In all KD patients, the patients treated with IV1G in 5th-10th day of illness has the least cardiac complication and CAL incidence and the group with IVIG therapy of 1 g/kg twice also has the least cardiac complication and CAL incidence. Conclusions The best doses of IVIG in treating KD is 1 g/kg twice and the IVIG therapy should be used in 5th-10th day of KD illness.

Evaluation of the structure of tricuspid valve with three dimensional echocardiography in hypoplastic right ventricle / 中华超声影像学杂志

Objective To reveal the character of tricuspid valve in hypoplastic right ventricular heart syndrome (HPRVHS) using 3-dimensional echocardiography(3DE). Methods Twenty eight patients with HPRVHS were included in the study, and they were divided into two groups according the degree of tricuspid regurgitation. Eighteen normal children were included as the control. Volume of right ventricle(VRV), area of tricuspid annulus(TVA),proportion of the leaflets and the distance from tips of leaflets to the nearest papillary muscle were measured with 3DE. These data was standardized by body surface area. Results The volume of right ventricle in HPRVHS was significantly less than that in normal group. The area of tricuspid annulus was significantly correlated with volume of right ventricle(r = 0. 90, P < 0. 0001). In the tricuspid regurgitation group, the proportion of area of anterior leaflet to area of tricuspid annulus was significantly higher than non-regurgitation group and normal group(P <0. 0001). Respectively,the proportion of the area of posterior leaflet to the area of tricuspid annulus in regurgitation group was significantly less than normal and non-regurgitation group. The distance from tips of leaflets was significantly shorter in HPRVHS than in normal group. Furthermore, the distance from the tip of septal leaflet to the nearest papillary muscle was shorter in regurgitation group than non-regurgitation group ( P = 0. 0023). Conclusions 3DE could be applied for evaluating the pathologic change of tricuspid valve in congenital heart disease. In HPRVHS, hypoplastic of posterior leaflet and shortness of the cords of septal leaflet could be the cause of tricuspid regurgitation.

Study on three-dimensional echocardiography for quantifying left ventricular function in conotruncal defects / 中华超声影像学杂志

Objective To evaluate the accuracy and value of ventricular function in conotruncal defects(CTD)by three-dimensional echocardiography(3DE).Methods Fifty-two children with CTD and forty-three children with normal hearts were examined by 3DE.The measurements of left ventricular volume and wall mass were outlined and calculated.The measurements of left ventricular volume and wall mass of 3DE in preoperative CTD were compared with those of 2D biplane Simpson-method in preoperative CTD,3DE in control group and 2D biplane Simpson-method in control group.The results of 3DE and 2DE preoperative measurements were also compared with their postoperative clinical cardiac conditions.Results From the findings of analysis of variance and Student-Newman-Keuls(SNK)test,there was no significant difference between 3DE and 2DE measurements of left ventricular function in normal children,but there was significant difference between 3DE and 2DE measurements of left ventricular function in children with CTD.2DE measurements of left ventricular volume and wall mass in the CTD group were significant underestimated and less correlated with their postoperative clinical cardiac conditions(r=0.20,P=0.2086)than 3DE measurements(r=0.39,P=0.0090).Furthermore,left ventricular function in the CTD group was poorer than that in the control group.Stroke volume and effective function were reduced due to increasing end-systolic volume.while left ventricular wall mass was increased due to the possible compensation of its ventricular muscle.With the lower end-diastolic volume,stroke volume and effective function,patients were more likely to have the occurrence of postoperative low cardiac output.Conclusions By making a precise quantitative assessment,3DE is very useful in providing more information for preoperative diagnosis in CTD and predicting such postoperative prognoses as the occurrence of postoperative low cardiac output.